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Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 224-229 doi: 10.1007/s11705-008-0029-9

Abstract: The cell-free protein synthesis system provides a new strategy of protein expression with advantagessystem are the key factors for the successful construction of cell-free protein expression system.In this work, the cell extract was prepared from RNase I- defective strain A19.The cell growth phase, the pressure for cell disruption and the storage condition of cell extracts wereUnder the optimized conditions, the green fluorescent protein (GFP) reporter gene was expressed in the cell-free

Keywords： cell-free     regeneration     extract     information     successful construction    

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MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things Article

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Engineering 2022, Volume 8, Issue 1,   Pages 96-107 doi: 10.1016/j.eng.2021.11.002

Abstract: Thus, we can build a non-terrestrial network (NTN) using a cell-free architecture.time-sensitive requirements and uneven distribution of IoT devices, the NTN must be empowered using mobileedge computing (MEC) while providing oasisoriented on-demand coverage for devices.

Keywords： Cell-free Mobile edge computing     Non-terrestrial networks     Sixth-generation     Wide-area time-sensitive    

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Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 58-65 doi: 10.1007/s11705-017-1610-x

Abstract: The advent of synthetic biology has ushered in new applications of cell-free transcription-translationThese cell-free systems are reconstituted using cellular proteins, and are amenable to modular controlHere, we discuss the historical advancement of cell-free systems, as well as their new applications inFinally, we present our vision on the future direction of cell-free synthetic biology.

Keywords： cell-free system     application    

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Edge Computing Technology: Development and Countermeasures

Hong Xuehai and Wang Yang

Strategic Study of CAE 2018, Volume 20, Issue 2,   Pages 20-26 doi: 10.15302/J-SSCAE-2018.02.004

Abstract:

Edge computing is an emerging technology that reduces transmission delaysMobile terminals, Internet of things, and other devices provide the necessary front-end support for computingcomputing.This paper discusses the concept of edge computing, key problems that require solutions, main advancesin edge computing, influence of edge computing developments, and opportunities and development countermeasures

Keywords： cloud computing     edge computing     fog computing     mobile edge computing     internet of things     front-end    

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Energy-efficient trajectory planning for a multi-UAV-assisted mobile edge computing system

Pei-qiu Huang, Yong Wang, Ke-zhi Wang,pqhuang@csu.edu.cn,ywang@csu.edu.cn,kezhi.wang@northumbria.ac.uk

Frontiers of Information Technology & Electronic Engineering 2020, Volume 21, Issue 12,   Pages 1671-1814 doi: 10.1631/FITEE.2000315

Abstract: We study a system assisted by (UAVs), where the UAVs act as edge servers to provide computing services
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Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 522-527 doi: 10.1007/s11684-017-0526-7

Abstract: Detecting circulating tumor cell (CTC) or circulating tumor DNA (ctDNA) in plasma or serum could serve

Keywords： liquid biopsy     circulating tumor cells     cell-free ctDNA     exosomes     precision medicine    

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Cost-effective resource segmentation in hierarchical mobile edge clouds Special Feature on Future Network-Research Article

Ming-shuang JIN, Shuai GAO, Hong-bin LUO, Hong-ke ZHANG

Frontiers of Information Technology & Electronic Engineering 2019, Volume 20, Issue 9,   Pages 1209-1220 doi: 10.1631/FITEE.1800203

Abstract: ., edge caching and edge computing) at the network edge.., cache placement strategy and vCPU provision strategy) for edge applications from the perspective ofthere is no literature that focuses on how to efficiently allocate resources from the perspective of a mobile

Keywords： Edge clouds     Edge computing     Edge caching     Resource segmentation     Virtual machine (VM) allocation    

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Real-time task processing method based on edge computing for spinning CPS

Shiyong YIN, Jinsong BAO, Jie LI, Jie ZHANG

Frontiers of Mechanical Engineering 2019, Volume 14, Issue 3,   Pages 320-331 doi: 10.1007/s11465-019-0542-1

Abstract: This paper presents an edge computing-based method that is different from traditional centralized cloudEdge computing nodes, real-time tasks, and edge computing resources are defined.A system model is established, and a real-time task processing method is proposed for the edge computingExperimental results indicate that the proposed real-time task processing method based on edge computing

Keywords： edge computing     real-time task     scheduling     CPS     spinning    

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Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 526-532 doi: 10.1007/s11783-010-0261-7

Abstract: cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free

Keywords： microcystin-LR(MC-LR)     biodegradation     Sphingopyxis sp     USTB-05     cell-free extract    

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Analyzing the service availability of mobile cloud computing systems by fluid-flow approximation

Hong-wu LV,Jun-yu LIN,Hui-qiang WANG,Guang-sheng FENG,Mo ZHOU

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 7,   Pages 553-567 doi: 10.1631/FITEE.1400410

Abstract: Mobile cloud computing (MCC) has become a promising technique to deal with computation- or data-intensiveIt overcomes the limited processing power, poor storage capacity, and short battery life of mobile devices

Keywords： Service availability     Mobile cloud computing     Fluid-flow approximation     Ordinary differential equations    

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Development and Prospect of Edge Intelligence for Industrial Internet

Ren Yaodanjun, Qi Zhengwei, Guan Haibing, Chen Lei

Strategic Study of CAE 2021, Volume 23, Issue 2,   Pages 104-111 doi: 10.15302/J-SSCAE-2021.02.014

Abstract: Edge intelligence, a product of edge computing and artificial intelligence, completes intelligent tasksusing computing resources near the data origin.This study reviews the research status of cooperative computing, resource isolation, privacy protection, and other key technologies in edge intelligence.Moreover, the development trend of edge intelligence for the industrial Internet is analyzed in terms

Keywords： industrial Internet，edge computing，edge intelligence，cooperative computing，resource isolation    

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Should medium-sized contractors still implement home-made information technologies on construction sites?

Conrad BOTON, Éric ST-PIERRE, Gabriel LEFEBVRE

Frontiers of Engineering Management 2020, Volume 7, Issue 1,   Pages 142-158 doi: 10.1007/s42524-019-0065-9

Abstract: Despite the increasing dissemination of the Building Information Modeling (BIM) approach, many firms are still far from BIM and are only starting to adopt information technologies. We conducted an ethnographic action research with an industrial partner that implements information technologies on its construction sites, with a strong preference for home-made applications. Results show that the development of such applications faces difficulties and that multiple iterations and versions are necessary before achieving the expected goals because the required competencies are lacking among internal development teams. Moreover, initial needs evolve considerably throughout the application’s development and evaluation process. However, notwithstanding the difficulties encountered during the different application development phases, the industrial partner believes the decision to be worthwhile because of the desire to have internal control over the project data and the need to consider certain specificities related to business processes.

Keywords： small- and medium-sized enterprises     mobile computing     mobile cloud computing     information technology for    

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Flexible Resource Scheduling for Software-Defined Cloud Manufacturing with Edge Computing Article

Chen Yang,Fangyin Liao,Shulin Lan,Lihui Wang,Weiming Shen,George Q. Huang

Engineering 2023, Volume 22, Issue 3,   Pages 60-70 doi: 10.1016/j.eng.2021.08.022

Abstract: Subsequently, edge computing is introduced to complement the cloud with computation and storage capabilities

Keywords： Cloud manufacturing     Edge computing     Software-defined networks     Industrial internet of things     Industry    

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Formation of free-charging industry alliance for new energy vehicles

Zhengdong YANG, Feng JIN, Shiyu DU, Jingwen LI

Frontiers of Engineering Management 2018, Volume 5, Issue 2,   Pages 268-275 doi: 10.15302/J-FEM-2018058

Abstract: The advanced charging technology of Internet-distributed mobile energy can link up with many market participantsunexpected multiple goals, for example, (1) consumers who have purchased new energy vehicles can avail free

Keywords： industry alliance     mobile energy     new energy vehicles     free charging     low carbon    

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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering Article

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Engineering 2018, Volume 4, Issue 6,   Pages 888-893 doi: 10.1016/j.eng.2018.09.007

Abstract: The enzymatic activities of the R61A, L88F, and I179F mutants, which were obtained with a rapid cell-free

Keywords： Polyesterase     PET degradation     Cell-free protein synthesis     Polyester     PETase    

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Title Author Date Type Operation

Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Journal Article

MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Journal Article

Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Journal Article

Edge Computing Technology: Development and Countermeasures

Hong Xuehai and Wang Yang

Journal Article

Energy-efficient trajectory planning for a multi-UAV-assisted mobile edge computing system

Pei-qiu Huang, Yong Wang, Ke-zhi Wang,pqhuang@csu.edu.cn,ywang@csu.edu.cn,kezhi.wang@northumbria.ac.uk

Journal Article

Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Journal Article

Cost-effective resource segmentation in hierarchical mobile edge clouds

Ming-shuang JIN, Shuai GAO, Hong-bin LUO, Hong-ke ZHANG

Journal Article

Real-time task processing method based on edge computing for spinning CPS

Shiyong YIN, Jinsong BAO, Jie LI, Jie ZHANG

Journal Article

Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Journal Article

Analyzing the service availability of mobile cloud computing systems by fluid-flow approximation

Hong-wu LV,Jun-yu LIN,Hui-qiang WANG,Guang-sheng FENG,Mo ZHOU

Journal Article

Development and Prospect of Edge Intelligence for Industrial Internet

Ren Yaodanjun, Qi Zhengwei, Guan Haibing, Chen Lei

Journal Article

Should medium-sized contractors still implement home-made information technologies on construction sites?

Conrad BOTON, Éric ST-PIERRE, Gabriel LEFEBVRE

Journal Article

Flexible Resource Scheduling for Software-Defined Cloud Manufacturing with Edge Computing

Chen Yang,Fangyin Liao,Shulin Lan,Lihui Wang,Weiming Shen,George Q. Huang

Journal Article

Formation of free-charging industry alliance for new energy vehicles

Zhengdong YANG, Feng JIN, Shiyu DU, Jingwen LI

Journal Article

Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Journal Article